EXPLORATION OF HYBRIDIZATION AS A PROCESS OF SPECIATION AMONG THE SPECIES DROSOPHILA VIRILIS, D. MOJAVENSIS & D. PSEUDOOBSCURA JILLYN COOK Biology and Chemistry Department, Lake Superior State University, Sault Ste. Marie, Michigan 49783, USA Abstract. The possibility of hybridization as a factor of speciation was explored by experimenting with three different Drosophila species; D. virilis, D. mojavensis, and D. pseudoobscura. Data was collected January 11 through February 26, 1993. Methods for care of the flies were adapted from Carolina Drosophila Manual 1988. Six different hybrid crosses were attempted by using a no-choice method in order to restrict reproductive isolation. Objectives were to obtain F1 hybrids, test them for fertility, and examine possible mechanisms for success and failure. No hybrids were obtained. Several deaths were caused by two types of mold; Rhizopus and Mucor. Many of the adults died without successful crossing. Mechanisms for the failure of this experiment were considered to be caused by experimental error, for example, contamination; sexual selection factors, such as courtship behaviors; and isolating mechanisms, for instance, potential mates meet but do not mate. SELECTED REFERENCES Hoikkala, A. 1986. The maintenance of sexual isolation between four sympatric species of the Drosophila virilis group. Anim. Behav. 34:158-161. Koepfer, R.H. 1987. Selection for sexual isolation between geographic forms of Drosophila mojavensis I. interactions between the selected forms. Evolution 41:37-48. Levin, Donald A. 1979. Hybridization an evolutionary perspective. Dowden, Hutchingson and Ross Inc, Stroudsburg, Pennsylvania , USA.