Lake Superior State University
Lake Superior State University
 
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Alum Success

"I chose to transfer to Lake Superior State University because of the personal attention I would receive. I did not want to attend a large university where I would be a number lost in the masses. The well-rounded curriculum allowed me to obtain my position at John Deere. Having a good balance of theory and hands-on knowledge is critical for my position and Lake State gave me a good foundation in these skills."

Maria DiValentin, Mechanical Engineering 2001
Field Service Representative,
John Deere Power Systems
Waterloo, IA

School of Engineering

Senior Projects 2007-2008

 

Team ORD Logo

Team Off Road Designs

Members:

  • Charles Birbeck
  • Matthew Brehmer
  • Jason Filek
  • Anthony Fleming
  • John Geukes
  • Jacob Hauser
  • Christopher Parlowe

Faculty Advisor:

  • Jon Coullard

Company:

  • Lake Superior State University-SAE

Industrial Contact:

  • Paul Duesing

Project Description: Mini-Baja Vehicle Development

The key goals for Team ORD were to design and build a lightweight, fast, and strong mini Baja vehicle to compete at the Baja SAE Illinois Competition in June. The competition will consist of several dynamic and static events that will test the performance of the vehicle. Dynamic events measure the acceleration, handling, and endurance. Static events measure the quality of the design, safety and costs.

Links to the 2007-2008 Teams:

Optim- ization of Salmon DNA as an Internal Standard for qPCR

Elaina Murray

The Escherichia coli species is a human fecal contamination indicator and as such is used in beach monitoring efforts. Quantifying E. Coli presence in local beach waters helps the health department determine if a beach should be closed. The current method of determination, Colilert, takes 18 hours to produce data. Quantitative Polymerase Chain Reaction (qPCR), which measure genetic DNA, is also method used to quantify the number of E. Coli, but it can be done much faster than Colilert. In order to standardize the qPCR results, an internal standard is included which is salmon DNA. This project goes through the process of optimizing the salmon standard curve. Each of the components was modified and the resulting standard curve was analyzed for improvements; the primers and probe were purchased new and the concentrations were varied, the DNA was purchased new and the standard curve concentrations and dilution methods were varied, the DNA was cleaned with a Qiagen kit, and new master mix and bovine serum albumin were purchased and prepared. We found that changes to the concentrations of primers and probe and cleaning the DNA showed an increase of optimization, and that changing the dilution methods had no effect of optimization. A combination of the above modifications may be able to produce an optimized salmon DNA standard curve.

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