Lake Superior State University
Lake Superior State University
 
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Alum Success

Rachael graduated from Novi High School in Novi, Michigan. Rachael completed their senior research project by developing a method of detecting blastomyces dermatitidis in soil using PCR. This study lays the foundation for further studying blastomyces dermatitidis in soil samples from endemic regions in northern Michigan. Rachael is a member of the Alpha Chi Honor Society. Rachael recently was selected to go to MSU to train in qPCR techniques and assisted in their cow bacteroides project. Rachael plans to attend graduate school in the future to study biochemistry.

Rachael Cunningham
2010 Outstanding Graduate
Forensic Chemistry

Department of Environmental Sciences

Degree Audits

The following Degree Audits are for our environmental programs effective Spring 2014.

NOTE: Historical degree audits

 

Character- ization of Yellow Pigments in Freshwater Flavo- bacteria

Ashley Ryckman

The yellow color of Flavobacteria is due to the presence of carotenoids and flexirubin-type pigments. These complex chemical structures have shown to produce antioxidant properties, as well as antibacterial activity. The KOH test and reverse-phase high performance liquid chromatography (HPLC) was used to characterize carotenoid and flexirubin pigment production in three strains of Flavorbacteria: FR 87, FR Y, and FR 93. Optimization of pigment and separation was performed by quant-prep HPLC. Separation of four pigment fractions from each strain was attained using semi-prep HPLC. Antibacterial activity of the pigment fractions was tested using a MTT Assay. Fexirubin biosynthesis genes, darA and darB, of related pigment producing Flavobacteria were used to compare flexirubin pigments in FR 87. The three strains were determined to produce the carotenoid, Zeaxanthin. FR 87 produced 13 flexirubin pigments, FR Y produced 14 flexirubin pigments and FR 93 produced 20 flexirubin-type pigments. It was determined that compounds in all three strains demonstrated antibacterial activity. The flexirubin biosynthesis gene, darA, is 89.9% similar to Flavobacterium johnsoniae, and 73.2% similar to Flavobacterium psychrophilum.

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