Bootcamp for NMR Educators
Schedule (Updated 5/2/2011)
Location: Crawford Hall of Science
Directions and Accommodations
Monday, May 23rd
- 8:45am - Registration Closes for students (Only $10 – includes lunch!).
- 9:00-11:30am - Keynote Presentation
- Joseph Hornak (Rochester Institute of Technology)
“Teaching NMR Fundamentals using the web and other new tools.” Abstract Below.
- 11:30am-12:30pm - Lunch
- 12:30-3:00pm - Keynote Presentation
- John Glushka (University of Georgia, Complex Carbohydrate Research Center)
“Teaching NMR by examining Pulse Sequences.” Abstract Below.
- 3:30-5:30pm - Soo Locks Boat Tour (2 hrs)
- 6:00-7:30pm – Dinner – Antlers Restaurant.
- 7:30pm-??? - Evening Mixer: Research Idea Exchange, Dessert and Refreshments.
Tuesday, May 24th
- 9:00-11:30am - Morning Session: Research and Educational Talks
- Dean Antic (PicoSpin) “Making NMR Accessible: picoSpin-45 NMR spectrometry in the Classroom.” Abstract Below.
- Clemens Anklin (Bruker Biospin Corp.) “Remote and virtual NMR.” Abstract Below.
- Marshall Werner (Lake Superior State University) “The use of 31P NMR as a tool to demonstrate enzyme kinetics.” Abstract Below.
- Charles Abrams (Truman College)“Beyond NMR Tutor: Teaching Spectroscopy in a Studio Classroom.” Abstract Below.
- 11:30am-12:30pm – Lunch
- 12:30-2:30pm - Afternoon Session: Research and Educational Talks
- Don Bouchard (Anasazi Instruments)
“Uncommon uses for NMR in various disciplines.”
Abstract Pending.
- Marshall Werner (Lake Superior State University)
“Pulse Sequence Guide for the Anasazi- a potential teaching tool.”
Abstract Below.
- Room for more talks and presentations so please register and submit your abstract ASAP.
Abstracts So Far…(If you plan on attending, consider submitting an abstract; we would all like to hear what you have to say about NMR.)
Joseph Hornak (Rochester Institute of Technology)
“Teaching NMR Fundamentals using the web and other new tools.”
Abstract: Nuclear magnetic resonance (NMR) is unique among spectroscopies introduced to undergraduates in that it is a resonance technique. As such, NMR concepts are more difficult to understand than absorption concepts. Compounding this problem is the dynamic nature of constructs used to explain NMR, thus static diagrams used in textbooks are not a good media for conveying these dynamic principles. HTML code with animated GIF images is well suited for solving this problem. The Basics of NMR is an HTML educational package created to overcome the static diagram problem by incorporating animated GIF images to introduce the spin physics of NMR. It is an open-access, web-based, self-paced educational text on NMR. The evolution of the sample magnetization during excitation and relaxation processes is easily described. The approach is continued through several pulse sequences. The general educational procedure used in The Basics of NMR will be presented, along with a brief history of its development.
John Glushka (University of Georgia, Complex Carbohydrate Research Center)
“Teaching NMR by examining Pulse Sequences.”
Abstract: NMR data can be used successfully in a straightforward analytical fashion, matching spectral fingerprints or relying on characteristic signals to identify compounds. Even higher dimensional data can be analyzed simply as patterns of signals that are consistent with a structure. However, much of the power of NMR comes from its ability to correlate signals using different physical phenomena. Modern pulse programs are designed to exploit those correlations and can provide not only the primary chemical structure, but also the three dimensional conformation and motional behavior of a molecule. Analyzing pulse programs can be useful as way to understand the origins of the data, and thus differentiate between desired signals and artifacts. Pulse programs provide tools to calibrate the spectrometer in order to optimize data collection, and they can be used to illustrate concepts of quantum or RF physics. If one is applying NMR to probe some molecular feature or activity such as ligand binding, it is essential to understand which experiments are suitable and which parameters should be varied. We will go over basic 1D and 2D pulse sequences for the homo- and heteronuclear experiments that are generally used for primary structure elucidation (e.g. COSY, TOCSY, NOESY, ROESY, HSQC, HMBC). If there is interest and time, we can also consider techniques such as selective pulses and touch on elements of hyphenated- and higher dimensional combinations of these experiments (e.g. HSQC-TOCSY) that are being applied to biomolecules.
Dean Antic (PicoSpin)
“Making NMR Accessible: picoSpin-45 NMR spectrometry in the Classroom.”
Abstract: The picoSpin-45 NMR spectrometer is a 45 MHz permanent magnet spectrometer incorporating a low volume capillary cartridge probe, high resolution digital pulse sequencer, embedded web server and browser based GUI for instrument control, providing high-resolution spectra (< 100 ppb) in a portable, compact design. Measuring a 1H NMR spectrum to determine product purity, monitor the course of a chemical reaction, or elucidating molecular structure is a valuable skill best acquired through hands-on experience as it personalizes the process for students, making it a much more meaningful learning experience, and remains a powerful teaching method for instructors. The lower cost and small footprint of miniature and bench-top instrumentation brings NMR to the student, in the classroom or lab, providing increased accessibility to a broader group of students. The picoSpin-45 spectrometer has applications in organic, physical and analytical chemistry teaching labs and classrooms, from basic spectroscopic concepts such as multiplet patterns and spin-spin coupling, to more advanced applications of chemical reaction dynamics and measuring spin-lattice relaxation (T1) delays.
Marshall Werner (Lake Superior State University)
“The use of 31P NMR as a tool to demonstrate enzyme kinetics.”
Abstract: Undergraduates in chemistry are routinely exposed to both 1H and 13C NMR spectroscopy but may not have an opportunity to work with other NMR active nuclei. 31P NMR offers an excellent way to do this because of its relatively high magnetogyric ratio and 100% natural abundance both of which allow students to acquire a spectrum in a short period of time. Students studying biochemistry become familiar with phosphate containing compounds such as adenosine triphosphate (ATP), phosphoenol pyruvate (PEP), and creatine phosphate (CP). Traditionally, they are shown how to assay for these compounds using an enzyme coupled assay monitoring the indirect production or disappearance of NADH or NAD+ or similar UV active compound. As an alternative, the direct measurement of phosphate containing compounds by 31P NMR will be presented with a focus on enzymatic catalysis within the glycolytic pathway.
Charles Abrams (Truman College)
“Beyond NMR Tutor: Teaching Spectroscopy in a Studio Classroom”
Abstract: Students who participate in undergraduate research at the City Colleges of Chicago are introduced to NMR theory, instrument operation, and sampling techniques during a three-hour workshop offered once per year. Teaching these workshops in our Studio Classroom (faculty.ccc.edu/cabrams/studio) allowed us to incorporate demonstrations, hands-on instrument operation, sample preparation, data analysis, and animations with NMR Tutor (www.nmrtutor.com), all in the same room for 32 students and faculty. During one workshop, students used a water suppression pulse sequence to measure carbohydrate, fat, and protein in samples of milk. Students posed questions about setting various parameters, which were answered immediately by running the instrument and collecting data during the workshop. The studio classroom made it easy for students to view sample preparation using a document camera, prepare their own samples, and have a front seat at the NMR console for all participants simultaneously by controlling the Anasazi EFT-60 via Windows Remote Desktop.
Clemens Anklin (Bruker Biospin Corp.)
“Remote and virtual NMR.”
Abstract: Various ways of operating a spectrometer over the internet will be presented. Methods such as SSH, VNC and remote desktop and others will be discussed. How can sample changers and webcams change the way students or entire small institutions get access to instrumentation. The virtual NMR part will deal with software packages that can allow students to explore NMR without using magnet time.
Donald Bouchard (Anasazi Instruments)
“Uncommon uses for NMR in various disciplines.”
Abstract: Pending.
Marshall Werner (Lake Superior State University)
“Pulse Sequence Guide for the Anasazi NMR- a potential teaching tool.”
Abstract: A concise guide to the Anasazi pulse program language will be presented. Several detailed pulse sequences will be examined along with potential modifications to help students understand the actions that both the software and instrument are performing during an experiment. Conference attendee will receive both a paper and electronic copy of this Guide.
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