Lake Superior State University
Lake Superior State University
 
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Alum Success

Video at ABC.com

Ariel Kelly, a Park and Recreation graduate who is a park ranger at Yosemite National Park, was interviewed as part of a program feature that highlights beautiful places in America.

Parks & Recreation

Parks & Recreation Management challenges the mind and body
Scholarships
 
One of Seven Chosen...

Russell D. Bruce Recreation Department Scholarship

This scholarship honors Dr. Russell D. Bruce, the first department head of recreation disciplines at LSSU. Awarded to a recreation or exercise science major at the end of their junior year, selection is based on academics, leadership, and service contribution to the Recreation Club or Exercise Science Professional Club.

  

Brandon Fast, of Honor, MI, graduated with a degree in Parks & Recreation management from LSSU in May and will apply his $1,000 scholarship toward an internship this summer that is a part of his degree requirements. His internship is with the U.S. Forest Service in Mio, MI.

"This is a great tribute to our program," said Prof. Deb McPherson, chair of the department.

Brandon worked six summers as an interpreter at the Platte River State Fish Hatchery. A student member of MPRA, Fast worked as a volunteer at the organization's state convention in 2007.

Brandon received one of seven scholarships offered to students throughout the state.

NOTE: MPRA is a state association of professionals who are working in recreation, leisure, parks and sports-affiliated careers. The organization provides continuing education, certifications, workshops and an annual conference for members. It staffs representatives in Lansing to discuss issues important to its members with the state legislature.

 

Optim- ization of Salmon DNA as an Internal Standard for qPCR

Elaina Murray

The Escherichia coli species is a human fecal contamination indicator and as such is used in beach monitoring efforts. Quantifying E. Coli presence in local beach waters helps the health department determine if a beach should be closed. The current method of determination, Colilert, takes 18 hours to produce data. Quantitative Polymerase Chain Reaction (qPCR), which measure genetic DNA, is also method used to quantify the number of E. Coli, but it can be done much faster than Colilert. In order to standardize the qPCR results, an internal standard is included which is salmon DNA. This project goes through the process of optimizing the salmon standard curve. Each of the components was modified and the resulting standard curve was analyzed for improvements; the primers and probe were purchased new and the concentrations were varied, the DNA was purchased new and the standard curve concentrations and dilution methods were varied, the DNA was cleaned with a Qiagen kit, and new master mix and bovine serum albumin were purchased and prepared. We found that changes to the concentrations of primers and probe and cleaning the DNA showed an increase of optimization, and that changing the dilution methods had no effect of optimization. A combination of the above modifications may be able to produce an optimized salmon DNA standard curve.

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