Lake Superior State University
Lake Superior State University
 
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Alum Success

Communications and SPC Administrator, Drinking Source Water Protection Division of the Sault Ste. Marie Region Conservation Authority (Ontario)

"Most natural science classes at LSSU offer students as much hands-on experience (lab hours) as lecture hours, which ensures that the university experience prepares us for the real world careers, whether you work at a desk or out in the field, or both. There are endless advantages and opportunities available to LSSU students due to its location on an international border.

I found my career path on campus when my advisor posted a job for the Bi-national Public Advisory Council (BPAC), a group dedicated to and overseeing the clean-up the St. Mary's River. I was able to gain experience and network on both sides of the border, ultimately finding full-time employment before I graduated with my biology degree."

Loralei Premo '07
Biology Major

Prelaw

Careers*
   

Attorney — Quite simply, attorneys practice law. What that means depends upon the legal (or non-legal) work environment that one ultimately chooses, along with the area(s) of law in which one practices. The opportunities are endless given the various work environments and the numerous areas of law in which one can specialize. For instance, lawyers practice within private law practices; public interest groups; governmental agencies (federal, state, tribal or local); courts; business and industry (e.g., insurance companies, financial institutions, corporations, hospitals, public relation firms, political campaigns, labor unions, and trade associations); academics (as a law professor, law librarian or administrator); or in various non-legal careers such as the media, law enforcement, business, public relations, foreign service, or politics. In addition, within many of these varied work environments, there are numerous areas of law in which one may specialize.

Career Choices:

  • Trial Lawyer
  • Corporate Counsel
  • Prosecuting Attorney
  • Judge or Magistrate
  • Law School Professor or Administrator
  • Mediator or Arbitrator
  • Federal or State Legislator
  • Public Defender

 

  

 

Optim- ization of Salmon DNA as an Internal Standard for qPCR

Elaina Murray

The Escherichia coli species is a human fecal contamination indicator and as such is used in beach monitoring efforts. Quantifying E. Coli presence in local beach waters helps the health department determine if a beach should be closed. The current method of determination, Colilert, takes 18 hours to produce data. Quantitative Polymerase Chain Reaction (qPCR), which measure genetic DNA, is also method used to quantify the number of E. Coli, but it can be done much faster than Colilert. In order to standardize the qPCR results, an internal standard is included which is salmon DNA. This project goes through the process of optimizing the salmon standard curve. Each of the components was modified and the resulting standard curve was analyzed for improvements; the primers and probe were purchased new and the concentrations were varied, the DNA was purchased new and the standard curve concentrations and dilution methods were varied, the DNA was cleaned with a Qiagen kit, and new master mix and bovine serum albumin were purchased and prepared. We found that changes to the concentrations of primers and probe and cleaning the DNA showed an increase of optimization, and that changing the dilution methods had no effect of optimization. A combination of the above modifications may be able to produce an optimized salmon DNA standard curve.

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