Lake Superior State University
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Alum Success

“I chose LSSU expecting a very good engineering education. What I didn’t expect was faculty with real-world engineering experience and abilities, labs with real-world equipment, projects with real-world outcomes, and an entire campus staff with real interest in my success, as a student and yet today. My LSSU engineering education has created or supported every desired career opportunity. LSSU was absolutely the right place for me.”

Dan Goodrich,
Mechanical Engineering 1999,
Vehicle Test & Development,
Electronic Brake Systems Group

School of Engineering

Senior Projects 2010-2011

 

PT

Team PROTOTECH

 

Team PT Group Picture  

PDC Projects & LSSU Lab Refurbishment

Members:

  • Adam Ball
  • Sean De Carlo
  • Mark Rodriguez
  • Steven Solack

Faculty Advisor:

  • Dr. Robert Hildebrand

Company:

  • Lakes Superior State University and the LSSU Product Development Center

Industrial Contact:

  • Mr. Eric Becks, Mr. David Leach, Dr. Mansoor Janjua, and
    Dr. Sai Nudurupati

Project Description: PDC Projects & LSSU Lab Refurbishment

Team ProtoTech used its resources to develop prototype devices for several of the Product Development Center’s clients in addition to refurbishing the School’s thermal- fluids laboratory. Tasks included research, development, troubleshooting, construction and evaluation of custom and proprietary parts and systems. The projects made full use of the team’s diverse knowledge base of in-depth mechanical and electrical design work.

Links to the 2010-2011 Teams:

Character- ization of Yellow Pigments in Freshwater Flavo- bacteria

Ashley Ryckman

The yellow color of Flavobacteria is due to the presence of carotenoids and flexirubin-type pigments. These complex chemical structures have shown to produce antioxidant properties, as well as antibacterial activity. The KOH test and reverse-phase high performance liquid chromatography (HPLC) was used to characterize carotenoid and flexirubin pigment production in three strains of Flavorbacteria: FR 87, FR Y, and FR 93. Optimization of pigment and separation was performed by quant-prep HPLC. Separation of four pigment fractions from each strain was attained using semi-prep HPLC. Antibacterial activity of the pigment fractions was tested using a MTT Assay. Fexirubin biosynthesis genes, darA and darB, of related pigment producing Flavobacteria were used to compare flexirubin pigments in FR 87. The three strains were determined to produce the carotenoid, Zeaxanthin. FR 87 produced 13 flexirubin pigments, FR Y produced 14 flexirubin pigments and FR 93 produced 20 flexirubin-type pigments. It was determined that compounds in all three strains demonstrated antibacterial activity. The flexirubin biosynthesis gene, darA, is 89.9% similar to Flavobacterium johnsoniae, and 73.2% similar to Flavobacterium psychrophilum.

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