Lake Superior State University
Lake Superior State University
 
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Alum Success

23 Michigan Conservation Officers - all LSSU alumni - met up at Camp Grayling

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Criminal Justice
Outstanding reputation for producing well educated and well trained criminal justice practitioners
Senior Projects
 
Study Challenge?

As part of the program, Criminal Justice students will present a senior thesis. An academic challenge such as this, is not available to an undergraduate student at many other colleges and universities. Students have found that preparing their thesis also helped them narrow down their career focus and allowed them to be better prepared for job placement opportunities.

As part of the academic requirements, senior internship (typically taken during the summer between the junior and senior years) is also an important asset to future employment success. Our students often are able to intern with law enforcement agencies that they wish to work for. A few have had unique opportunities such as county marine patrol, Customs and Immigration, the court system, prosecutor’s offices and the like.

  

A strong program in a demanding field

The criminal justice is now demanding more academic preparation from the people employed in this field.

Optim- ization of Salmon DNA as an Internal Standard for qPCR

Elaina Murray

The Escherichia coli species is a human fecal contamination indicator and as such is used in beach monitoring efforts. Quantifying E. Coli presence in local beach waters helps the health department determine if a beach should be closed. The current method of determination, Colilert, takes 18 hours to produce data. Quantitative Polymerase Chain Reaction (qPCR), which measure genetic DNA, is also method used to quantify the number of E. Coli, but it can be done much faster than Colilert. In order to standardize the qPCR results, an internal standard is included which is salmon DNA. This project goes through the process of optimizing the salmon standard curve. Each of the components was modified and the resulting standard curve was analyzed for improvements; the primers and probe were purchased new and the concentrations were varied, the DNA was purchased new and the standard curve concentrations and dilution methods were varied, the DNA was cleaned with a Qiagen kit, and new master mix and bovine serum albumin were purchased and prepared. We found that changes to the concentrations of primers and probe and cleaning the DNA showed an increase of optimization, and that changing the dilution methods had no effect of optimization. A combination of the above modifications may be able to produce an optimized salmon DNA standard curve.

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